0373700 20240111140813.920120312235959.9 000310729400054 10.5220/0003797703470350 4 s. CD cav_un_epca*0373699978-989-8425-90-4347-350AlgarveSciTePress - Science and Technology Publications2012SchierJanCorreiaCarlosFredAnaGamboaHugo FISH analysis Fluorescence microscopy Image analysis Image segmentation cav_un_auth*0101190 Schier Jan Zpracování obrazové informace Department of Image Processing ZOI ZOI UTIA-B Department of Image Processing Ústav teorie informace a automatizace AV ČR, v. v. i. cav_un_auth*0101136 Kovář Bohumil Zpracování obrazové informace Department of Image Processing ZOI ZOI UTIA-B Ústav teorie informace a automatizace AV ČR, v. v. i. cav_un_auth*0275271 Kočárek E. CZ PDF soubor http://library.utia.cas.cz/separaty/2012/ZOI/schier-automated detection of interphase and metaphase nuclei in the fish images.pdf TA01010931 GA TA ČR cav_un_auth*0272418 CEZ:AV0Z10750506 The fluorescence in-situ hybridization (FISH) belongs to the most common cytogenetic methods and is widely applied in routine clinical genetic diagnostics. We are paying attention to FISH analysis of chromosomal aneuploidies – the deviations from chromosomal number. Such analysis is based on evaluation of up to several hundreds of microscopic images. Computer support for this process includes using methods of image processing and data mining. In this paper, we focus on the image processing part in more detail: first, the properties of FISH images are reviewed, then, the processing flow is outlined. Our aim is to find the interphase and metaphase nuclei and the hybridization signals contained in the image. A simple method using the raw and central moments of detected objects as measures to distinguish between the two types of nuclei is proposed. cav_un_auth*0279363 BIOINFORMATICS 2012, International Conference on Bioinformatics Models, Methods and Algorithms Vilamoura, Algarve 01.02.2012-04.02.2012 PT 2012 JC 3 PR http://hdl.handle.net/11104/0206773 2012 000310729400054 WOS PDF soubor http://library.utia.cas.cz/separaty/2012/ZOI/schier-automated detection of interphase and metaphase nuclei in the fish images.pdf cav_un_epca*0373699 BIOINFORMATICS 2012, Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms 978-989-8425-90-4 347 350 Algarve SciTePress - Science and Technology Publications 2012 Schier Jan 340 Correia Carlos 340 Fred Ana 340 Gamboa Hugo 340