0435026 20240103205032.020141120235959.9 6 s. P cav_un_epca*04350251-901725-51-0185-190LondonCity University London2014Reyes-AldasoroConstantino CarlosSlabaughGreg image segmentation phase contrast microscopy time-lapse imaging cav_un_auth*0293859 Soukup Jindřich Zpracování obrazové informace Department of Image Processing ZOI ZOI UTIA-B Department of Image Processing Ústav teorie informace a automatizace AV ČR, v. v. i. cav_un_auth*0309796 Lašan M. CZ cav_un_auth*0101209 Šroubek Filip Zpracování obrazové informace Department of Image Processing ZOI ZOI UTIA-B Department of Image Processing Ústav teorie informace a automatizace AV ČR, v. v. i. http://library.utia.cas.cz/separaty/2014/ZOI/soukup-0435026.pdf LO1205 GA MŠk CZ cav_un_auth*0301046 134/2013/Z GAJU CZ cav_un_auth*0309797 914813/2013 GA UK CZ cav_un_auth*0309798 GA13-29225S GA ČR cav_un_auth*0292734 CZ.1.05/2.1.00/ 01.0024 OP VaVpI CZ cav_un_auth*0313923 Phase contrast microscopy is widely used for live cell imaging. Automatic computer processing of phase-contrast images is complicated, particularly because of the halos that are present around the cells. We introduce a new algorithm able to segment individual cells by using the halos. The halos between cells are detected by thresholding and skeletonization, and gaps in the edges are filled in using Dijkstra’s algorithm. The result of the algorithm was compared with the results of manual segmentation performed by a human expert and evaluated on level of precision of individual cells’ edges. The source code and test data, including the manual segmentation are made available to the public. cav_un_auth*0309554 Medical Image Understanding and Analysis 2014 London 09.07.2014-11.07.2014 GB 2015 JD 3 PO RVO:67985556 http://hdl.handle.net/11104/0239120 cav_un_auth*0296001 MFF UK Univerzita Karlova v Praze, Matematicko-fyzikální fakulta CZ cav_un_auth*0300331 Jihočeská univerzita v Českých Budějovicích, Fakulta rybářství a ochrany vod CZ S 2014 cav_un_epca*0435025 Proceedings of the 18th Conference on Medical Image Understanding and Analysis 1-901725-51-0 185 190 London City University London 2014 Reyes-Aldasoro Constantino Carlos 340 Slabaugh Greg 340