0597935 20250331101450.520240905235959.9 85198332961 001270312300001 10.1016/j.isci.2024.110451 15 s. E cav_un_epca*0497635iScience27Cell Press Aurora kinase A mouse oocyte meiotic spindles cav_un_auth*0409371 Blengini C. S. US cav_un_auth*0356104 Vaškovičová Michaela UZFG-Y Laboratoř integrity DNA Laboratory of DNA Integrity LID Biomedical Research Team CZ Ústav živočišné fyziologie a genetiky AV ČR, v. v. i. cav_un_auth*0101190 Schier Jan UTIA-B Zpracování obrazové informace Department of Image Processing ZOI ZOI Department of Image Processing Ústav teorie informace a automatizace AV ČR, v. v. i. cav_un_auth*0329873 Drutovič Dávid UZFG-Y Laboratoř integrity DNA Laboratory of DNA Integrity LID Biomedical Research Team CZ Ústav živočišné fyziologie a genetiky AV ČR, v. v. i. cav_un_auth*0340757 Schindler K. US https://www.sciencedirect.com/science/article/pii/S2589004224016766?via%3Dihub GA23-07532S GA ČR CZ cav_un_auth*0458771 Meiotic spindles are critical to ensure chromosome segregation during gamete formation. Oocytes lack centrosomes and use alternative microtubule-nucleation mechanisms for spindle building. How these mechanisms are regulated is still unknown. Aurora kinase A (AURKA) is essential for mouse oocyte meiosis because in pro-metaphase I it triggers microtubule organizing-center fragmentation and its expression compensates for the loss of the two other Aurora kinases (AURKB/AURKC). Although knockout mouse models were useful for foundational studies, AURK spatial and temporal functions are not yet resolved. We provide high-resolution analyses of AURKA/AURKC requirements during meiotic spindle-building and identify the subcellular populations that carry out these functions: 1) AURKA is required in early spindle assembly and later for spindle stability, whereas 2) AURKC is required in late pro-metaphase, and 3) Targeted AURKA constructs expressed in triple AURK knockout oocytes reveal that spindle pole-localized AURKA is the most important population controlling spindle building and stability mechanisms. WOS 10000 10600 10605 2025 UTIA-B 10000 10200 10201 4 A 4a 20250106102525.6 RVO:67985904 RVO:67985556 https://hdl.handle.net/11104/0355715 S CC BY-NC-ND license open access 110451 A MULTIDISCIPLINARYSCIENCES 4.7 0.9 2.8 0.08854 1.397 41252 97 1.363 67.21 4.21 29167 Q1 4.000 2765 Q1 84.2 0.94 Q1 84.2 2024 Soubory v repozitáři: Blengini_Vaskovicova_2024_iScience.pdf 85198332961 SCOPUS 39081293 PUBMED 001270312300001 WOS cav_un_epca*0497635 iScience 2589-0042 Roč. 27 č. 8 2024 Cell Press ONLINE