| bibtype |
J -
Journal Article
|
| ARLID |
0355844 |
| utime |
20240111140752.9 |
| mtime |
20110308235959.9 |
| WOS |
000282750300009 |
| DOI |
10.1093/glycob/cwq105 |
| title
(primary) (eng) |
The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase |
| specification |
|
| serial |
| ARLID |
cav_un_epca*0256673 |
| ISSN |
0959-6658 |
| title
|
Glycobiology |
| volume_id |
20 |
| volume |
11 (2010) |
| page_num |
1410-1419 |
| publisher |
| name |
Oxford University Press |
|
|
| keyword |
α-N-acetylgalactosaminidase |
| keyword |
α-galactosidase |
| keyword |
Aspergillus niger |
| keyword |
substrate binding |
| keyword |
molecular modeling |
| author
(primary) |
| ARLID |
cav_un_auth*0269444 |
| name1 |
Kulik |
| name2 |
Natallia |
| full_dept (cz) |
Oddělení struktury a funkce proteinů |
| full_dept (eng) |
Department of Structure and Function of Proteins |
| institution |
UEK-B |
| fullinstit |
Ústav výzkumu globální změny AV ČR, v. v. i. |
|
| author
|
| ARLID |
cav_un_auth*0270141 |
| name1 |
Weignerová |
| name2 |
L. |
| country |
CZ |
|
| author
|
| ARLID |
cav_un_auth*0241929 |
| name1 |
Filipi |
| name2 |
Tomáš |
| full_dept (cz) |
114 - Laboratoř biotrasformací |
| full_dept |
114 - Laboratory of Biotransformation |
| institution |
MBU-M |
| fullinstit |
Mikrobiologický ústav AV ČR, v. v. i. |
|
| author
|
| ARLID |
cav_un_auth*0104712 |
| name1 |
Pompach |
| name2 |
Petr |
| full_dept (cz) |
113 - Laboratoř charakterizace molekulární struktury |
| full_dept |
113 - Laboratory of Molecular Structure Characterization |
| institution |
MBU-M |
| full_dept |
Laboratory of Structural Biology and Cell Signalization |
| fullinstit |
Mikrobiologický ústav AV ČR, v. v. i. |
|
| author
|
| ARLID |
cav_un_auth*0265032 |
| name1 |
Novák |
| name2 |
Petr |
| full_dept (cz) |
Stochastická informatika |
| full_dept |
Department of Stochastic Informatics |
| department (cz) |
SI |
| department |
SI |
| institution |
UTIA-B |
| fullinstit |
Ústav teorie informace a automatizace AV ČR, v. v. i. |
|
| author
|
| ARLID |
cav_un_auth*0269630 |
| name1 |
Mrázek |
| name2 |
H. |
| country |
CZ |
|
| author
|
| ARLID |
cav_un_auth*0247364 |
| name1 |
Slámová |
| name2 |
Kristýna |
| full_dept (cz) |
114 - Laboratoř biotrasformací |
| full_dept |
114 - Laboratory of Biotransformation |
| institution |
MBU-M |
| full_dept |
Laboratory of Biotransformation |
| fullinstit |
Mikrobiologický ústav AV ČR, v. v. i. |
|
| author
|
| ARLID |
cav_un_auth*0016491 |
| name1 |
Bezouška |
| name2 |
K. |
| country |
CZ |
|
| author
|
| ARLID |
cav_un_auth*0104590 |
| name1 |
Křen |
| name2 |
Vladimír |
| full_dept (cz) |
114 - Laboratoř biotrasformací |
| full_dept |
114 - Laboratory of Biotransformation |
| institution |
MBU-M |
| full_dept |
Laboratory of Biotransformation |
| fullinstit |
Mikrobiologický ústav AV ČR, v. v. i. |
|
| author
|
| ARLID |
cav_un_auth*0106027 |
| name1 |
Ettrich |
| name2 |
Rüdiger |
| full_dept (cz) |
Oddělení struktury a funkce proteinů |
| full_dept |
Department of Structure and Function of Proteins |
| institution |
UEK-B |
| fullinstit |
Ústav výzkumu globální změny AV ČR, v. v. i. |
|
| cas_special |
| project |
| project_id |
LC06010 |
| agency |
GA MŠk |
| country |
CZ |
| ARLID |
cav_un_auth*0219067 |
|
| project |
| project_id |
GAP207/10/1934 |
| agency |
GA ČR |
| ARLID |
cav_un_auth*0263478 |
|
| project |
| project_id |
GAP207/10/1040 |
| agency |
GA ČR |
| ARLID |
cav_un_auth*0263331 |
|
| project |
| project_id |
GA303/09/0477 |
| agency |
GA ČR |
| ARLID |
cav_un_auth*0252876 |
|
| project |
| project_id |
GAP207/10/0321 |
| agency |
GA ČR |
| ARLID |
cav_un_auth*0263330 |
|
| research |
CEZ:AV0Z60870520 |
| research |
CEZ:AV0Z50200510 |
| research |
CEZ:AV0Z10750506 |
| abstract
(eng) |
Two genes in the genome of Aspergillus niger, aglA and aglB, have been assigned to encode for α-d-galactosidases variant A and B. However, analyses of primary and 3D structures based on structural models of these two enzymes revealed significant differences in their active centers suggesting important differences in their specificity for the hydrolyzed carbohydrates. To test this unexpected finding, a large screening of libraries from 42 strains of filamentous fungi succeeded in identifying an enzyme from A. niger CCIM K2 that exhibited both α-galactosidase andα-N-acetylgalactosaminidase activities, with the latter activity predominating. The enzyme protein was sequenced, and its amino acid sequence could be unequivocally assigned to the enzyme encoded the aglA gene. Enzyme activity measurements and substrate docking clearly demonstrated the preference of the identified enzyme for α-N-acetyl-d-galactosaminide over α-d-galactoside. |
| reportyear |
2011 |
| RIV |
CE |
| num_of_auth |
10 |
| mrcbC52 |
4 A 4a 20231122134433.4 |
| permalink |
http://hdl.handle.net/11104/0194522 |
| mrcbT16-e |
BIOCHEMISTRYMOLECULARBIOLOGY |
| mrcbT16-f |
4.268 |
| mrcbT16-g |
1.213 |
| mrcbT16-h |
6.5 |
| mrcbT16-i |
0.01679 |
| mrcbT16-j |
1.399 |
| mrcbT16-k |
6010 |
| mrcbT16-l |
141 |
| mrcbT16-s |
1.829 |
| mrcbT16-4 |
Q1 |
| mrcbT16-B |
59.514 |
| mrcbT16-C |
65.909 |
| mrcbT16-D |
Q2 |
| mrcbT16-E |
Q1 |
| arlyear |
2010 |
| mrcbTft |
\nSoubory v repozitáři: Glycobiology_Final.pdf |
| mrcbU34 |
000282750300009 WOS |
| mrcbU56 |
1575 |
| mrcbU63 |
cav_un_epca*0256673 Glycobiology 0959-6658 1460-2423 Roč. 20 č. 11 2010 1410 1419 Oxford University Press |
|